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简要描述:产品简介:产品品牌:AnaSpec产品名称:SensoLyte®520凝血mei活性测定试剂盒荧光法-1试剂盒英文名称:SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit产品货号:AS-72129产品规格:1 kitAnaSpec 凝血mei活性测定试剂盒荧光法
品牌 | AnaSpec | 货号 | AS-72129 |
---|---|---|---|
规格 | 1 kit | 供货周期 | 现货 |
AnaSpec AS-72129 SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit,SensoLyte®520凝血mei活性测定试剂盒荧光法-1试剂盒
我们北京云肽生物科技有限公司作为AnaSpec公司的经销商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
AnaSpec ( 美国 )
AnaSpec公司成立于1993年,是一家为全球生命科学研究,提供综合蛋白质组学和基因组学解决方案的领xian供应商。
公司产品主要分三大部分:提供专业多肽,检测试剂,抗体检测试剂盒;适合基础研究的高通量筛选和新药物研发相关试剂;多肽合成,抗体生产,寡核苷酸的合成等定制服务。
AnaSpec 凝血mei活性测定试剂盒荧光法
AnaSpec AS-72129 SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit,SensoLyte®520凝血mie活性测定试剂盒荧光法-1试剂盒,产品简介:
产品品牌:AnaSpec
产品名称:SensoLyte®520凝血mei活性测定试剂盒荧光法-1试剂盒
英文名称:SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit
产品货号:AS-72129
产品规格:1 kit
AnaSpec AS-72129 SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit,SensoLyte®520凝血mei活性测定试剂盒荧光法-1试剂盒,产品说明:
Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion. The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity. This kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate for thrombin. Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors.
凝血mei是一种丝氨suan蛋白酶,通过将可溶性血浆纤维蛋白原转化为不溶性纤维蛋白凝块和促进血小板聚集,在止血中发挥中心作用。凝血mei还影响许多正常和病理过程,包括炎症、组织修复、胚胎发生、血管生成和肿瘤侵袭。SensoLyte®520凝血mei分析试剂盒经过优化,可用于检测凝血mei活性。该套件包含一种新型内部淬火5-FAM/QXL™ 520凝血mei的FRET底物。凝血mei将FRET底物切割成两个单独的片段,导致5-FAM荧光的释放,可在激发/发射=490 nm/520 nm时进行监测。5-FAM的长波荧光受生物样品和供试化合物中成分的自发荧光干扰较小。该分析可在96孔或384孔微孔板中进行,以筛选酶抑制剂。
Component A: 5-FAM/QXL™520 Thrombin substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: 5-FAM, Fluorescence reference standard, Ex/Em=490 nm/520 nm: 2 mM, 10 µL Component C: 2X Assay buffe:r 20 mL Component D: Purified human thrombin enzyme: 0.1 mg/mL, 40 µL Component E: Thrombin inhibitor: 60 µM, 10 µL Component F: Stop solution: 5 mL
组件A:5-FAM/QXL™520凝血mei底物,Ex/Em=490 nm/520 nm切割后:2 mM,50µL组分B:5-FAM,荧光参考标准,Ex/Em=490 nm/520 nm:2 mM,10µL组分C:2X分析缓冲液:r 20 mL组分D:纯化人凝血mei:0.1 mg/mL,40µL组分E:凝血mei抑制剂:60µM,10µL组分F:停止液:5 mL
AnaSpec 凝血mei活性测定试剂盒荧光法
产品订购信息
AnaSpec AS-72129 SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit,SensoLyte®520凝血mei活性测定试剂盒荧光法-1试剂盒 1 kit
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