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010-62817090Assay Biotechnology 重组蛋白和合成肽
Assay Biotechnolog公司自2006年1月成立以来,Assay Biotechnology一直是业界领 xian 的抗体生产和工程和测定技术,荧光染料,猝灭剂,重组蛋白和合成肽的全球贡献者。位于加利福尼亚州旧金山湾区的心脏地带。
在我们成立后的头几年,Assay Biotech成立了抗体制造商和抗体工程公司,通过OEM合作伙伴关系专门针对大型公司。从那时起,我们一直在成为一个突出的测定和抗体来源,将其覆盖范围扩大到全球超过50个国家的数百万研究人员。
我们一直在寻求推动我们潜力的信条,决心成为业界的测定开发和制造公司之一,提供大量即用型测定,免疫试剂,分子检测模块,生物标志物和全线抗体工程服务支持zui艰巨的学术,药学和临床研究工作。
协议:
将所有试剂制备至工作浓度,将标准品制备至所需范围,并将样品制备至适当的稀释因子。
取出所需数量的捕获抗体包被条进行实验,并将剩余的条带放回装有干燥剂的干燥袋中,进行 4°C 储存。
向每个孔中加入100μl标准品/样品,并在室温(RT)下在轨道振荡器上孵育2小时。
吸出溶液,向每个正在使用的孔中加入300μl1x洗涤缓冲液,并在轨道振荡器上轻轻振荡2-3分钟。重复此过程 3 次。最后一次洗涤后,通过倒置盘子并将其敲击在干净的纸巾上,确保没有液体残留。
向每个孔中加入 100 μl 1x 检测抗体并孵育 2 小时。在 RT 的轨道振动台上
重复步骤 4。
向每个孔中加入100μl1x SAV-HRP,并在室温轨道振荡器上孵育30分钟
重复步骤 4。
向每个孔中加入100μl复合增强剂/过氧化物溶液,盖板免受光照,并在室温轨道振荡器上孵育5分钟。
在光度计上读取板。
基于比色的细胞ELISA
基于比色的基于细胞的 ELISA 试剂盒可检测各种靶蛋白以及某些刺激条件对不同细胞系中靶蛋白表达的影响。通过间接ELISA形式实现目标蛋白浓度的定性测定。
从本质上讲,靶蛋白被靶标特异性一抗 (1°) 捕获,而 HRP 偶联的二抗 (2°) 结合 1° 抗体的 Fc 区域。通过这种结合,与 2° 抗体偶联的 HRP 酶可以在添加底物时催化比色反应。
由于基于细胞的 ELISA 的定性性质,描述了多种归一化方法:1) 包括对人 GAPDH 具有特异性的单克隆抗体,作为归一化目标吸光度值的内部阳性对照。2)通过添加底物对HRP活性进行比色测量后,采用结晶紫全细胞染色法测定细胞密度。
染色后,可以通过将吸光度值归一化为细胞量来分析结果,从而可以调整镀层差异。3) 如果检测到磷酸化靶标,将提供针对非磷酸化对应物的抗体用于标准化目的。获得的非磷酸化靶标的吸光度值可用于归一化磷酸化靶标的吸光度值。
下面是Assay Biotechnology公司的部分热销产品
产品名称 | 货号 | 规格 |
Tryptophan Hydroxylase (Phospho-Ser260) Antibody | P12-1295-100 | 100 ug |
HSL (Phospho-Ser855/554) Antibody | A0074-50 | 50 ug |
PFKFB2 (Phospho-Ser483) Antibody | A0761-50 | 50 ug |
eNOS (Phospho-Ser615) Antibody | A1145-50 | 50 ug |
PYK2 (Phospho-Tyr402) Antibody | A7204-50 | 50 ug |
SREBP-1 (Phospho-Ser439) Antibody | A8425-50 | 50 ug |
SP1 Antibody | B0577-50 | 50 ug |
Tryptophan Hydroxylase Antibody | B1011-50 | 50 ug |
IRS-1 Antibody | B7120-50 | 50 ug |
Paxillin Antibody | B8250-50 | 50 ug |
OCT2 Antibody | C0282-50 | 50 ug |
HARS Antibody | C10349-50 | 50 ug |
CGK 2 Antibody | C10961-50 | 50 ug |
IRX2 Antibody | C11651-50 | 50 ug |
Collagen IX alpha3 Antibody | C12212-50 | 50 ug |
ABHD12 Antibody | C14209-50 | 50 ug |
ST6GAL1 Antibody | C15170-50 | 50 ug |
AASDHPPT Antibody | C16447-50 | 50 ug |
APOBEC3D/F Antibody | C17872-50 | 50 ug |
SFRS15 Antibody | C18932-50 | 50 ug |
THY1 Antibody | C21278-50 | 50 ug |
ITGAD Antibody | C30328-50 | 50 ug |
SAMHD1 Antibody | C30986-50 | 50 ug |
GNRHR Antibody | G276-50 | 50 ug |
OR6K2 Antibody | G663-50 | 50 ug |
Caspase 6 (Cleaved-Asp162) Antibody | L0106-50 | 50 ug |
S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody | P12-1174-50 | 50 ug |
Lyn Antibody | R12-2230-50 | 50 ug |
ANGPTL7 Antibody | R12-2486-50 | 50 ug |
EIF4E3 Antibody | R12-2742-50 | 50 ug |
MARK3 Antibody | R12-2998-50 | 50 ug |
OR6P1 Antibody | R12-3254-50 | 50 ug |
SIRT3 Antibody | R12-3510-50 | 50 ug |
eEF2K (Phospho-Ser366) Antibody | A0071-100 | 100 ug |
14-3-3 thet/tau (Phospho-Ser232) Antibody | A0758-100 | 100 ug |
Catenin-alpha1 (Phospho-Ser641) Antibody | A1140-100 | 100 ug |
PTEN (Phospho-Ser370) Antibody | A7201-100 | 100 ug |
Gab2 (Phospho-Ser623) Antibody | A8350-100 | 100 ug |
p70 S6 Kinase Antibody | B0534-100 | 100 ug |
HER3 Antibody | B0946-100 | 100 ug |
FKHRL1 Antibody | B7087-100 | 100 ug |
RSK1/2/3/4 Antibody | B8182-100 | 100 ug |
Keratin 10 Antibody | C0249-100 | 100 ug |
MOS Antibody | C10258-100 | 100 ug |
Mevalonate Kinase Antibody | C10868-100 | 100 ug |
TREF1 Antibody | C11526-100 | 100 ug |
CDC5L Antibody | C12171-100 | 100 ug |
PRKAG1 Antibody | C14134-100 | 100 ug |
CBLN2 Antibody | C15057-100 | 100 ug |
GBP3 Antibody | C16080-100 | 100 ug |
4E-BP1 Monoclonal Antibody | N0001 | 50ul |
A1BG Monoclonal Antibody | N0004 | 50ul |
AATM Monoclonal Antibody | N0006 | 50ul |
AIF-M1 Monoclonal Antibody | N0017 | 50ul |
YB1 (Phospho-Ser102) Antibody | P12-1200 | 50ul |
Tryptophan Hydroxylase (Phospho-Ser260) Antibody | P12-1195 | 50ul |
TIF-IA (Phospho-Ser649) Antibody | P12-1189 | 50ul |
STMN1 (Phospho-Ser62) Antibody | P12-1182 | 50ul |
MAP2K7 (Phospho-Thr275) Antibody | P12-1140 | 50ul |
LIMK2 (Phospho-Thr505) Antibody | P12-1137 | 50ul |
GRK2 (Phospho-Ser685) Antibody | P12-1112 | 50ul |
FLT3 (Phospho-Tyr969) Antibody | P12-1108 | 50ul |
FAK (Phospho-Tyr397) Antibody | P12-1103 | 50ul |
B-RAF (Phospho-Ser446) Antibody | P12-1080 | 50ul |
ATF1 (Phospho-Ser63) Antibody | P12-1077 | 50ul |
AhR (Phospho-Ser36) Antibody | P12-1072 | 50ul |
ATP-citrate synthase (Phospho-Ser455) Antibody | P12-1003 | 50ul |
ACK (Phospho-Tyr518) Antibody | P12-1001 | 50ul |
EGFR (Phospho-Thr693) Antibody | A0009 | 50ul |
HSP90β (Phospho-Ser254) Antibody | A0013 | 50ul |
ALK (Phospho-Tyr1586) Antibody | P12-1002 | 50ul |
Adducin α/β (Phospho-Ser726/713) Antibody | A0002 | 50ul |
GSK3α/β (Phospho-Tyr279/216) Antibody | A0012 | 50ul |
C/EBP α (Phospho-Ser21) Antibody | A0056 | 50ul |
Caspase-9 (Phospho-Thr125) Antibody | A0060 | 50ul |
eEF2K (Phospho-Ser366) Antibody | A0071 | 50ul |
Cyclin D1 (Phospho-Thr286) Antibody | A0417 | 50ul |
Goat Anti-Chicken IgY (H&L) - Cy7 | SA1257 | 50ul |
Goat Anti-Human IgG gamma chain - Cy7 | SA1261 | 50ul |
Goat Anti-Human IgM mu chain - Cy7 | SA1263 | 100 ul |
Goat Anti-Rabbit IgG (H&L) - Cy7 | SA1267 | 100 ul |
Rabbit Anti-Chicken IgY (H&L) - Cy7 | SA1270 | 100 ul |
Rabbit Anti-Guinea Pig IgG (H&L) - Cy7 | SA1275 | 100 ul |
Mouse Anti-Human Ig lambda chain - Cy7 | SA1297 | 100 ul |
Rabbit Anti-Human IgG F(ab')2 - Cy7 | SA1299 | 100 ul |
Rabbit Anti-Human IgG (H&L) - Cy7 | SA1277 | 100 ul |
Rabbit Anti-Human IgG gamma chain - Cy7 | SA1279 | 100 ul |
Rabbit Anti-Monkey IgG (H&L) - Cy7 | SA1282 | 100 ul |
Goat Anti-Chicken IgY (H&L) - AMCA | SA0440 | 100 ul |
Goat Anti-Human IgG Fab - AMCA | SA0442 | 100 ul |
Goat Anti-Human IgG+IgM+IgA - AMCA | SA0445 | 100 ul |
Goat Anti-Rabbit IgG (H&L) - AMCA | SA0450 | 100 ul |
Goat Anti Chicken IgY (H&L) - Alexa Fluor 750 | SA1042 | 100 ul |
Goat Anti Rabbit IgG (H&L) - Alexa Fluor 750 | SA1052 | 100 ul |
Rabbit Anti Dog IgG (H&L) - Alexa Fluor 750 | SA1056 | 100 ul |
Rabbit Anti Guinea Pig IgG (H&L) - Alexa Fluor 750 | SA1060 | 100 ul |
Rabbit Anti Human IgG Fc - Alexa Fluor 750 | SA1063 | 100 ul |
Rabbit Anti Human IgG+IgM+IgA - Alexa Fluor 750 | SA1065 | 100 ul |
Rabbit Anti Monkey IgG (H&L) - Alexa Fluor 750 | SA1067 | 100 ul |
Rabbit Anti Sheep IgG (H&L) - Alexa Fluor 750 | SA1071 | 100 ul |
Mouse Anti Human IgG Fab - Alexa Fluor 750 | SA1075 | 100 ul |
Mouse Anti Human IgM - Alexa Fluor 750 | SA1077 | 100 ul |
Mouse Anti Human Ig kappa chain - Alexa Fluor 750 | SA1081 | 100 ul |
BMAL1 (Acetyl-Lys538) Colorimetric Cell-Based ELISA Kit | CBA1086 | 1 Kit |
p73 (Acetyl-Lys327) Colorimetric Cell-Based ELISA Kit | CBA1085 | 1 Kit |
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